Intended Use
Brucella IgG/IgM Antibody Rapid Test kit (Immunochromatographic Assay) is suitable for the qualitative clinical screening of serum/plasma/whole blood samples for the detection of antibodies antibodies anti-Brucella. It is intended to be used as a screening test and as an aid in the diagnosis of infection with Brucella.
Test Principle
Brucella IgG/IgM Antibody Rapid Test kit (Immunochromatographic Assay) is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant Brucella antigen conjugated with colloid gold and rabbit IgG-gold conjugates 2) a nitrocellulose membrane strip containing two test bands (M and G bands) and a control band (C band). The M band is pre-coated with monoclonal antihuman IgM for the detection of anti- Brucella IgM, G band is pre-coated with reagents for the detection of anti- Brucella IgG, and the C band is pre-coated with goat anti rabbit IgG. When an adequate volume of test specimen.
Main Contents
Components provided are listed in the table.
| Component REF/REF |
B014C-01 |
B014C-05 |
B014C-001-25 |
| Test Cassette |
1 test |
5 tests |
25 tests |
| Buffer |
1 bottle |
5 bottles |
25 bottles |
| Dropper |
1 piece |
5 pcs |
25 pcs |
| Disposable Lancet |
1 piece |
5 pcs |
25 pcs |
| Specimen Transport Bag |
1 piece |
5 pcs |
25 pcs |
| Instructions for Use |
1 piece |
1 piece |
1 piece |
| Certificate of Conformity |
1 piece |
1 piece |
1 piece |
Operation Flow
Step 1: Sampling
Collect human Serum/Plasma/Whole blood properly.
Step 2: Testing
1. Remove an extraction tube from the kit and a test box from the film bag by tearing the notch. Put it on the horizontal plane.
2. Open the inspection card aluminum foil bag. Remove the test card and place it horizontally on a table.
3. Open the buffer tube by twisting off the top. Put 3 drops (about 100 μL) of diluent into the assay diluent well round shaped. Start counting.
Step 3: Reading
15 mins later, read the results visually. (Note: do NOT read the results after 20 mins!)
Result Interpretation
1. Negative result
If only the quality control line C appears and the detection lines G and M do
not show, it means that no Brucella antibody is detected and the result is
negative.
2. Positive Result
2.1 If both the quality control line C and the detection line M appear, it means
that the Brucella IgM antibody is detected, and the result is positive for the
IgM antibody.
2.2 If both the quality control line C and the detection line G appear, it means
that the Brucella IgG antibody is detected and the result is positive for the
IgG antibody.
2.3 If both the quality control line C and the detection lines G and M appear,
it means that the Brucella IgG and IgM antibodies are detected, and the result
is positive for both IgG and IgM antibodies.
3.Invalid Result
If the quality control line C cannot be observed, results will be invalid
regardless of whether a test line shows, and the test should be repeated.
Order Information
| Product Name |
Cat. No |
Size |
Specimen |
Shelf Life |
Trans. & Sto. Temp. |
| Brucella IgG/IgM Antibody Rapid test kit (Immunochromatographic Assay) |
B014C-001 |
1test/kit |
WSerum/Plasma/Whole Blood |
18 Months |
2-30℃ / 36-86℉ |
| B014C-05 |
5 tests/kit |
| B014C-25 |
25 tests/kit |